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SlackiaRANK: GenusTAXONOMY: Bacteria -> Terrabacteria group -> Actinobacteria -> Coriobacteriia -> Eggerthellales -> Eggerthellaceae -> Slackia OVERVIEW: 'Slackia' is a genus of Actinobacteria, in the family Coriobacteriaceae.Some strains of the genus Slackia also metabolize isoflavones . A Gram-positive, non-sporeforming bacterial strain, designated NATTS, was isolated from the fecal bacteria of an equol-producing individual. It converted daidzein to equol in cultures containing sorbose as carbon source . Using the 16S RNA sequence, the strain was determined to belong to Slackia . Further study showed that the numbers of Slackia sp. similar to strain NATTS in feces of Japanese men were correlated with the concentration of equol in the sera of both prostate cancer patients and controls. The genes converting daidzein to dihydrodaidzein, and dihydrodaidzein to equol, from this bacterium were cloned and characterized . They encoded three contiguous open reading frames (Orf 1–3) . Tsuji et al. showed that daidzein was converted to dihydrodaidzein by a protein encoded by orf3, belonging to the NADH:flavin oxidoreductase family. Dihydrodaidzein is converted to cis- and trans-tetrahydrodaidzein (THD) by a protein encoded by orf2, belonging to the short-chain dehydrogenase/reductase superfamily. cis and trans-THD are converted to equol by a protein encoded by orf1 that has an amino acid sequence similar to that of succinate dehydrogenase. This genus contains microbial species that can reside in the human gastrointestinal tract. [PMC 4262072]
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