The most important applications of OH come from its apparent antibacterial properties. Several studies have found OH to possess antibacterial and specifically antimycobacterial properties, which has a particular efficacy against Mycobacterium, the genus of bacteria that includes those that cause leprosy and tuberculosis in humans. Therefore, OH is most widely used to treat internal infections, particularly tuberculosis, among the indigenous. OH extract has shown to be effective in treating tuberculosis. It was identified that two polyynes, namely oplopandiol and falcarindiol are the main effective antimycobacterial constituents of the extract. Other polyynes, falcarinol, oplopandiol acetate, and (Z)-9,17-octadecadiene-12,14-diyne-1,11,16-triol 1-acetate, were also shown to be effective against both Mycobacterium tuberculosis and M. avium. In addition, the polyynes of OH extracts have shown to be effective against two Gram-positive bacteria S. aureus and B. subtilis, two Gram-negative bacteria E. coli DC2 and P. aeruginosa Z61, and the yeast Candida albicans. Falcarinol proved to be the most effective among the tested polyynes.