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Prevotella melaninogenicaRANK: SpeciesTAXONOMY: Bacteroidetes/Chlorobi group -> Bacteroidetes -> Bacteroidia -> Bacteroidales -> Prevotellaceae -> Prevotella -> Prevotella melaninogenica OVERVIEW: Identified as a constituent of the oral microbiome by Human Oral Microbiome Database. Identified as constituent of vaginal microbiome. [PMID:23282177] Prevotella melaninogenica is a gram-negative bacterium. Between outer and inner cell wall the membrane proteins are present in order to break down and hydrolyze the nutrients. Gram-negative bacterium has large periplasm where degradation of enzymes, attachment to proteins and impression of environment take place. The current studies have found that the enzyme that proceeds preteinase activity are located at the surface and within the P. melaninogenica. Prevotella malaninogenica hydrolyzes esculin but not starch. Esculin is blue florescent glucoside which gets hydrolyzed to glucose and esculetin (6,7-dihydroxycoumarin) due to heat and acid. This method can proceed also through amylolytic fermentation using ATP. This ATP is created through the process of substrate level phspholyration which generates ATP during catabolism and sets the membrane gradient. This organism does not reduce urease or nitrogen which could mean that P. melaninogenica only receives its nutrients from organic donor. This organism produces acetic, isobutyric and isovaleric acids. From this metabolism process, hydrolysis of esculin can be determined by measuring acid production from the fermentation of glucose and detection of esculetin by its reaction with iron which forms black colored pigments. This species has been identified as a resident in the human gastrointestinal tract based on the phylogenetic framework of its small subunit ribosomal RNA gene sequences.[PMC 4262072] |