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SubdoligranulumRANK: GenusTAXONOMY: Bacteria -> Terrabacteria group -> Firmicutes -> Clostridia -> Clostridiales -> Ruminococcaceae -> Subdoligranulum OVERVIEW: Cells are coccoid in shape but may show some pleomorphism. Gram-stain-negative. Nonmotile and nonspore-forming. Strictly anaerobic; no growth occurs in 2% oxygen. Cells do not survive heating at 80°C for 10 min. Catalase-negative. Glucose and some other carbohydrates are fermented. The major acid products formed in PYG broth are butyric and lactic acids together with minor amounts of acetic and succinic acids. Cells are indole-negative and nitrate is not reduced to nitrite. Esculin is hydrolyzed, but starch is not. Lecithinase-, urease-, and lipase-negative. Based on 16S rRNA gene sequence and phylogenetic analysis, this genus belongs to the Clostridium leptum rRNA super cluster (cluster IV). Isolated from human feces. A 16S rRNA-derived probe, Svariab_645: 5′-TGCACTACTCAAGGCCAG-3′, has been designed that specifically detects this genus by in situ hybridization to human fecal samples. DNA G+C content (mol%): 52.2. Type species: Subdoligranulum variabile This genus contains microbial species that can reside in the human gastrointestinal tract. [PMC 4262072]
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